Identification of the skin of Bufo bufo gargarizans and its adulterants based on DNA barcoding / 中国药学杂志

ABSTRACT

OBJECTIVE: To provide new technique for quick and accurate identification of the skin of Bufo bufo gargarizans u-sing DNA barcoding method based on the COI sequence. METHODS: Fifty-four samples of the skin of Bufo bufo gargarizans and its four kinds of adulterants were collected from 16 different localities. The DNAs were extracted, the COI squences were amplified and sequenced bi-directionally. The obtained sequences were assembled using the CodonCode Aligner V 4.2. Then the genetic distances were accumulated using Kimura-2-Parameter(K2P) model, and the NJ tree was constructed by MEGA5.0. RESULTS: The intra-specific genetic distances were much smaller than inter-specific ones between the skin of Bufo bufo gargarizans and its adulterants. Furthermore, the NJ tree showed that the skin of Bufo bufo gargarizans can be distinguished from its adulterants clearly. CONCLUSION: The COI sequence can be used as the DNA barcode to identify the skin of Bufo bufo gargarizans and its adulterants with accuracy and efficiency.