Role and molecular mechanisms of SET mediated pachtaxel resistance in MCF-7/PTX cells / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1390-1396, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-859593
ABSTRACT
OBJECTIVE:
To investigate the resistance mechanisms related to SET in paclitaxel-induced human breast cancer cells.METHODS:
The different expressions of SET and ABC transporters between MCF-7/S and paclitaxel resistant MCF-7/PTX cells were identified using Western blot. We adopted siRNA method to knockdown SET in MCF-7/PTX cells and plasmid transfection analysis to up-regulated SET in MCF-7/S cells. The cell viability to paclitaxel was assessed by MTT assay. The cell apoptosis was analyzed by flow cytometry. The levels of ABC transporters were analyzed using Western blot and Real-time PCR, respectively.RESULTS:
We found that higher levels of SET and ABC transporters in MCF-7/PTX cells. Knockdown of SET not only significantly sensitized MCF-7/PTX cells to paclitaxel, but also induced cell apoptosis. The levels of the ABC transporters were also reduced. Upregulated SET in MCF-7/S cells expressed resistant to paclitaxel and decreased cell apoptosis. High expression of the SET significantly promotes the mRNA and protein level of ABC transporters.CONCLUSION:
The above results demonstrate that SET is associated with paclitaxel resistance in MCF-7/PTX cells. The SET is expected to be one of novel biological targets of overcoming paclitaxel resistant in breast cancer treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2015
Type:
Article
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