Improvement of quality control method of liuwei dihuang pills / 中国药学杂志

ABSTRACT

OBJECTIVE: To improve the quality control method of Liuwei Dihuang Pills. METHODS: Morroniside and loganin from processed Fruetus Corni were identified by TLC. Morrniside, loganin and paeonol in Liuwei Dihuang Pills were determined by HPLC. The determination was performed on a Phenomenex Gemini 110A C18 column (4.6 mm×250 mm, 5 μm) maintained at 40℃ with gradient elution of acetonitrile (A) and 0.3% phosphoric acid aqueous solution (B) at a flow rate of 1 mL·min-1. The detective wavelength was set at 240 nm for morroniside and loganin and 274 nm for paeonol. RESULTS: The TLC spots were clear. Morrniside, loganin and paeonol showed good linear relationship in the range of 0.02078-1.039, 0.02034-1.017, and 0.04576-2.288 μg, respectively. The average recoveries (n=9) were 99.97%-100.99% with RSDs less than 2.3%. CONCLUSION: The method is simple, rapid, accurate and can be used to control the quality of Liuwei Dihuang Pill more effectively.