Regulation of Dickkopf (DKK1) on apoptosis of U937 cell / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the regulation of Dickkopf (DKK1) on apoptosis of human acute leukemia U937 cell. METHODS: The reactive oxygen species (ROS) was assessed with DCFH-DA fluorescence probe; the mitochondria membrane potential was assessed with JC-1 staining method, apoptosis rate was detected by AnnexinV-FITC/PI double staining, Western-blot was used to analyse the expression of related protein. RESULTS: Dickkopf can rise the ROS and reduce the mitochondria membrane potential, meanwhile up-regulate the expressing of BAX, down-regulating expressing of Mcl-1 and Bcl-2. CONCLUSION: Dickkopf can promote apoptosis of human acute leukemia U937 cell.