Effects of anti-inflammatory active fraction from the tuber of Scirpus yagaraohwi on mice with intracerebral haemorrhage / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the effects of anti-inflammatory active fraction (AF) from the tuber of Scirpus yagaraohwi in mice with intracerebral haemorrhage (ICH). METHODS: A 25 μL volume of autologous nonanticoagulated blood was collected from the tail artery of the mouse and then injected into the striatum using a syringe pump. Neurological deficits of the mouse was assessed 1, 3 and 5 days after ICH using a 28-point neurological deficit scale, and brain water content of the mouse cerebral tissues after ICH was measured at third day.The concentrations of pro-inflammatory cytokines in perihematoma tissues, including interleukin-6, interleukin-1β and tumor necrosis factor-α, were measured by ELISA assay. RESULTS: The results show that AF significantly reduced the neurological deficit scores and the brain water content after ICH. AF also markedly inhibited the levels of IL-6, IL-1β and TNF-α at first day and third day alter ICH. CONCLUSION: Anti-inflammatory active fraction of Scirpus yagaraohwi can improve the brain injury in mouse induced by ICH. The mechanism may involve decreased expression of pro-inflammatory cytokines of mouse after ICH.