Mechanism of broccoli polypeptide inhibited the growth of C6 glioma cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the inhibitory mechanism of broccoli polypeptide on the growth of C6 glioma cells of rats. METHODS: C6 glioma cells were cultured, the inhibitory of broccoli polypeptide on the growth of C6 glioma cells was examined by MTT assay. The morphology of C6 glioma cells was observed by inverted microscope. The cell cycle of C6 glioma cells was detected by flowcytometry. The expressions of bax, bcl-2 and caspase 3 proteins were detected by western blotting. RESULTS: The growth of C6 glioma cells was significantly inhibited after 10 μg·mL-1 broccoli polypeptide treatment at 48 h, 1 or 10 μg·mL-1 broccoli polypeptide treatment at 72 h, compared with control group (P<0.01, P<0.001). The density of C6 glioma cells was decreased according with the dose of broccoli polypeptide increased, the apoptotic bodies exist in broccoli polypeptide treated groups. The percentage of C6 glioma cells of SubG1 phase was increased, especially in 10 μg·mL-1 broccoli polypeptide group (P<0.01). The number of cells of G0/G1 phase and G2/M phase were decreased, whereas, S phase was increased. The ratio of bax/bcl-2 was increased in 1 and 10 μg·mL-11 broccoli polypeptide groups, and the expression of caspase 3 protein was increased in 10 μg·mL-1 broccoli polypeptide group, compared with control group (P<0.01, P<0.001). CONCLUSION: Broccoli polypeptide could inhibit the growth of C6 glioma cells through blocking cell cycle and activating apoptotic pathway.