Amelioration of diabetic nephropathy in rats by resveratrol: involvement of anti-inflammatory effect / 中国药学杂志

ABSTRACT

OBJECTIVE: To observe the protective effect of resveratrol (Rev) on diabetic nephropathy in rats with inflammation impairment. METHODS: Male rats were divided into two groups and fed with commercially available normal pellet diet (NPD) or in-house prepared high-fat diet (HFD), respectively. After 4 weeks of dietary feed, the rats from HFD group were injected intraperitoneally with low dose of streptozotocin (STZ) (40 mg·kg-1). Half of the rats from both groups were administered with a dose of Rev(50 mg·kg-1·d-1)for 32 weeks. The rats were allowed to continue to be feed on their respective diets until the end of the study. After 32 weeks, the body weight, levels of FPG, TC, TG, BUN, SCr, Cys C, and urine protein and kidney vascular permeability were assayed; the contents of IL-1β, IL-6 and ICAM-1 in serum were detected by ELISA; the vascular permeability of kidney was detected by Evans blue; glomerular morphological analysis was performed in PAS stained pathological section; the levels of ICAM-1 mRNA and the expression of ICAM-1 in kidney were detected by RT-PCR and Immunohistochemistry and Western blot. RESULTS: Rev had remarkably suppressed weight gain and lowered levels of FPG, TC, TG, BUN, SCr, Cys C, IL-1β, IL-6 and ICAM-1 in serum (P<0.05), decreased urine protein (P<0.05), and reduced kidney vascular permeability (P<0.05). Increased indices for glomerular injury were significantly ameliorated by Rev treatment (P<0.05). The expression of ICAM-1 mRNA was markedly up-regulated in the kidneys of diabetic rats, which was effectively suppressed by Rev treatment (P<0.05). Western blot and immunohistoehemistry analysis showed that the expression of ICAM-1 was reduced significantly by Rev treatment. CONCLUSION: Rev can ameliorate diabetic nephropathy damage in rats, and the protection role may be attributed to its effect to lower lipid and glucose levels in serum and inhibit inflammation in kidney.