Experimental study on the mechanism of anti-anaphylaxis effect of ethyl acetate extraction of Jing-fang powder / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigat the mechanism of anti-anaphylaxis effect of Jing-fang powder(traditional Chinese medicines) by using initiative systemic anaphylaxis model in mice. METHODS: The model of initiative systemic anaphylaxis in mice was induced by intraperitoneal injection with ovalbumin and DPT vaccine, and the ethyl acetate extraction of Jing-fang powder was intragastricly administrated for 14 d. ELISAs methods were used to measure the levels of total IgE and LTB4 in serum, and the levels of NO, iNOS and LTB4 in lung tissues in model mice. Ultraviolet spectrophotometry was used to measure the PGE2 level in lung tissue, and the index of thymus and spleen were also evaluated. RESULTS: At the dose of 6.67 g·g-1, the ethyl acetate extraction of Jing-fang powder decreased the index of thymus and spleen(P<0.05), as well as suppressed the production of total IgE and LTB4 in serum(P<0.05, and reduced the levels of NO, iNOS, LTB4 and PGE2 in lung tissue in model mice(P<0.05). Meanwhile, the extraction also suppressed the activity of iNOS in lung tissue under the dose of 6.67 and 10.01 g·g-1(P<0.05). CONCLUSION: These results suggest that the ethyl acetate extraction of Jing-fang Powder has potential anti-allergic effect, which the mechanism may be related to reduce the production of IgE, and inhibit the release of metabolic products of arachidonic acid, for example LTB4 and PGE2, and decrease the release of inflammatory allergy mediators NO.