Simultaneous determination of six index components in ligustri lucidi fructus by MEKC-DAD / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 994-998, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859713
ABSTRACT
OBJECTIVE:
To establish a micellar electrokinetic chromatography(MEKC) method for simultaneous determination of salidroside, tyrosol, ligustroflavone, specnuezhenide, oleanolic acid, and ursolic acid in Ligustri Lucidi Fructus.METHODS:
60 mmol·L-1 borax-10 mmol·L-1 SDS-30 mmol·L-1 hydroxypropyl-beta-cyclodextrin-10% methyl alcohol was used as the buffer solution (pH 9.03). Uncoated fused silica capillary (75 μm×64.5 cm, 56 cm of effective length) was used with separation voltage of 20 kV. The detection wavelength was set at 210 nm. The column temperature was maintained at 25°C, and the sample was injected at 5 kPa×6 s.RESULTS:
The calibration curves of the six index components showed good linearity (r>0.95) in the range of the tested concentrations, and the average recoveries of the method were between 94.57% and 102.07% (RSD<5%).CONCLUSION:
The method is simple, accurate and reproducible, and can be used for the quality control of Ligustri Lucidi Fructus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
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