Molecular identification of Angelica sinensis (oliv.) diels based on inter-simple sequence repeat (ISSR) analysis / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify Angelica sinensis and its adulterants by ISSR markers. METHODS: Genome polymorphism of 26 A. sinensis and its adulterants were analyzed by ISSR-PCR. The genetic similarity coefficients and genetic relationships were calculated by NTSYS-pc version 2.10. The dendrogram was constructed based on UPGMA clustering. RESULTS: Ten out of 100 ISSR primers are screened out and 96 bands were amplified, among which 85 were polymorphic fragments (percentage of polymorphic bands was 88.54%). The length of bands ranged from 200 to 2100 bp. One specific band was amplified by the primer UBC848.A. sinensis and its adulterants could be discriminated by primer UBC848 and UBC834. Results of UPGMA dendrogram indicated that A. sinensis from nine different places clustered together and related to A. acutiloba. The other species were clustered far away from A. sinensis. Three commercially crude materials purchased in the market were adulterants. CONCLUSION: The specific primers were found to identify A. sinensis by ISSR method. There is genetic diversity among A. sinensis from different production areas. The results provide molecular evidence for identification of A. sinensis and its adulterants and technical support of building up the stable quality evaluation system of A. sinensis.