Effect of Buyang Huanwu decoction on the denervated muscle atrophy of rats and investigation for mechanism / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the influence of buyang huanwu decoction (BYHWD) on the denervated tibial muscle atrophy of rats and to study the mechanisms of BYHWD defering denervated muscle atrophy of rats. METHODS: Sprague-Dawley rats were subjected to common peroneal nerve crush model, then they were randomly divided into sham operation group, model group, BYHWD high-dose, medium-dose, low-dose groups, mecobalamin group (positive control). Each group has ten rats. The gavage lasted for 18 d. After that, performed slice, staining and analysis morphology were performed. The wet weight ratio of tibial muscle was measured. At the same time, qRT-PCR and western-blot detect the differential expression of Angpt14 and PI3K in the high-dose group of BYHWD and model group. RESULTS: (1) Wet weight ratio BYHWD high and medium-group increased significantly compared with model group (P<0.01 or P<0.05). (2) The motor end-plate the sham operation group is sepia, oval or round; the BYHWD high-dose group and mecobalamin group has little chage; the BYHWD medium-dose group, low-dose group and the model group has lessen, shape is not rule gradually, the edge is rough, coloring is slight gradually, (3) the expression of Angpl14 and PI3K compared with model group, the BYHWD high and medium-group increased significantly (P<0.05 or P<0.01). CONCLUSION: BYHWD can effectively delay denervated tibial muscle atrophy of rats by increasing the wel weight ratio and defering the degeneration of the motor end-plate which mechanism maybe increase Angpt14 and PT3K expression.