Rapid determination of meropenem concentration in human plasma by column-switching-ultra high performance liquid chromatography / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 776-780, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859750
ABSTRACT
OBJECTIVE:
To establish a rapid column-switching-ultra high performance liquid chromatography method for determining meropenem concentration in human plasma.METHODS:
Meropenem plasma samples were diluted by internal standard aqueous solution (doxofylline), filtrated by microporous membrane, and then extracted by on-line column-switching method. The samples were chromatographed on Waters Acquity UPLC@BEH-C18 column using gradient elution method (mobile phase A and extracting mobile phase C methanol -0.05 mol·L-1 K2HPO4 (pH 7.0) 595; mobile phase B methanol). The maximum absorption wavelength of meropenem was 299 nm. The column temperature were 35°C. The meropenem concentration was calculated by internal standard method and external standard method, respectively. The results of the two methods were compared by paired t-test using SPSS statistical software.RESULTS:
The liner range of the calibration curve for meropenem was 0.67-86.00 mg·L-1 (r=0.9999). The method recovery was (93.59±1.15)%-(99.21±4.06)% and extraction recovery was above 85.0%, relative standard deviation was below 5.0%. The SPSS statistic results indicated there was no significant difference between the concentrations calculated by the two methods and existed good correlation. The transformed equation is ρInternal=1.039ρExternal-0.468 (r=0.9970).CONCLUSION:
On-line column-switching method is used and it is proved to be rapid, sensitive and suitable for therapeutic drug monitoring and pharmacokinetic investigation of meropenem in human plasma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
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