Preparation of heme from duck blood by a new acidified acetone extraction method / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare heme from cherry valley duck blood. METHODS: Single factor experiments and orthogonal experiment were carried out to establish the optimal process of preparing heme from cherry valley duck blood by a new microwave-assisted acid acetone extraction method. RESULTS: Single factor experiments proved that the microwave-assisted extraction process significantly improved the extraction efficiency of heme. In addition, orthogonal experiment indicated the power of microwave and microwave extraction time had significant effects on the extraction results, and the optimum conditions of microwave-assisted extraction in laboratory were as follows the power was 800 W, extraction time was 60 min, and the temperature was 50°C. CONCLUSION: The new microwave-assisted method, combining cell disruption and heme extraction process, can simplify the traditional acid acetone extraction process, and it is proved to be feasible.