Protection of puerarin on impaired vascular endothelial function induced by glycosylated protein / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 559-563, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859777
ABSTRACT
OBJECTIVE:
To investigate the effects of puerarin on impaired endothelium-dependent relaxation induced by glycosylated bovine serum albumin (GBSA) in rabbit thoracic aorta and its mechanisms.METHODS:
The rings were incubated with GBSA for 30 min to induce endothelial dysfunction, and with puerarin(0.25, 0.5 and 1 g · L-1), A-nitro-L-arginine methyl ester (L-NAME, 30 νmol · L-1), and indomethacin(10 μmol · L-1) to investigate the protective effect of puerarin on impaired vascular endothelial function elicited by GBSA, and the effect of L-NAME and indomethacin on the protective effect of puerarin. Moreover, the content of nitric oxide (NO) and malonaldehyde (MDA) and the activity of superoxide dismutase(SOD) in the rings were measured.RESULTS:
Exposure of aortic rings to GBSA (200 mg · L-1) for 30 min resulted in a significant inhibition of endothelium-dependent relaxation, but had no affect on endothelium-independent relaxation. GBSA significantly decreased the level of NO and the activity of SOD but hugely increased the content of MDA in vascular tissues. Pre-incubation of aortic rings with puerarin markedly attenuated the inhibition of endothelium-dependent relaxation induced by GBSA. This protective effect of puerarin (1 g · L-1) was partially inhibited by L-NAME but not by indomethacin. Puerarin obviously increased NO level and SOD activity but evidently decreased MDA content in rings.CONCLUSION:
Puerarin can protect against vascular endothelial dysfunction caused by GBSA and its mechanisms may be related to enhancing NO synthesis and its anti-oxidation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
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