Simultaneous determination of seven nucleosides in Isatidis Radix by HPCE / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a high performance capillary electrophoresis( HPCE) method for simultaneous determination of seven nucleosides, including epigoitrin, uracil, thymine, adenosine, hypoxanthine, guanosine and uridine in Isatidis Radix. METHODS: The chromatographic separation was performed on an uncoated fused silica capillary(75 μm × 64.5 cm, 56 cm of effective length) with separation voltage of 20 kV. 60 mmol · L-1 Na2B4O7-15 mmol · L-1 β-CD was selected as the running buffer solution(pH 9.6). The detection wavelength was set at 254 nm. The sample was injected at 5 kPa × 5 s and column temperature was maintained at 25°C. RESULTS: The calibration curves of the seven nucleosides showed good linearity (r > 0.9994). The average recoveries of the method were between 97.15%-100.40%. The contents of the seven nucleosides in Isatidis Radix samples of different batches were different. CONCLUSION: The established method is simple, accurate and can be used for the quality control of Isatidis Radix.