Isolation, identification and antimicrobial activities of endophytic fungi of Dysosma versipellis (Hance) M. Cheng / 中国药学杂志

ABSTRACT

OBJECTIVE: To examine the diversity and antimicrobial activities of the endophytic fungi of Dysosma versipellis (Hance) M. Cheng. METHODS: The identification of the fungi was carried out by rDNA-ITS sequence analysis. The antimicrobial activities of the isolated endophytic fungi were determined by agar block test. RESULTS: Nineteen strains of various endophytes were obtained from the roots, stems and leaves of D. versipellis, which were molecularly identified to belong to eight catalogues and thirteen genuses. The DV04 had remarkable antimicrobial activities to Staphylococcus aureus, Escherichia coli, and Candida albicans. Morphological and molecular identification demonstrated that DV04 represented Leptodontidium sp.. CONCLUSION: The endophytic fungi in Dysosma versipellis are diverse and rich. Some strains such as DV04 deserve further research as an alternative antimicrobial resource.