Study of degranulation induced by the chemical composition of polysorbate 80 in a rat mast (RBL-2H3) cell line / 中国药学杂志

ABSTRACT

OBJECTIVE: To study directive effects of eight types of the chemical composition of polysorbate 80 on the degranulation in the RBL-2H3 rat mast cell line and to illuminate the material basis for its pseudoallergy. METHODS: RBL-2H3 cells were cultured and treated with varying dosage of eight types of the chemical composition of polysorbate 80. And then the amount of the released β-hexosaminidase was detected. MTT assay was used to determine the cytotoxicity of eight types of the chemical composition of polysorbate 80 which causing RBL-2H3 cells degranulation. RESULTS: PSM, PSD and PIM induced RBL-2H3 cells' degranulation in a concentration-dependent manner. Degranulation was not detected in cells treated with PS and PI. PSTri, PSTetra and PSD weakly induced the degranulation of RBL-2H3 cells in a concentration-dependent manner. PSM, PSD and PIM showed cytotoxicity with concentration-dependent manner, while PS and PI had no cytotoxicity. PSTri, PSTetra and PSD showed low toxicity cytotoxicity in a concentration-dependent manner. CONCLUSION: PIM is a major source of toxic substances polysorbate 80, the limit of which must be controlled.