Regulation effect of isoniazid on the expression of hepatobiliary membrane transporters Mrp2, Bsep, P-gp and Ntcp in mouse / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 298-302, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859834
ABSTRACT
OBJECTIVE:
To investigate the regulation effect of isoniazid on the hepatobiliary membrane transporters multidrug resistance protein 2 (Mrp2), bile salt export pump (Bsep), P-glycoproteins (P-gp), sodium taurocholate cotransporting plypeptide (Ntcp), and which would lay the foundation for the studies of the mechanism on isoniazid-induced liver injury from the level of transporters.METHODS:
Following an oral dose of 30, 60, 120 mg · kg-1 · d-1 for 1, 2 and 3 months in mouse respectively, the biochemical indicator of serum were determined; the liver were removed for hepatic pathology; the protein mass of Bsep, Mrp2, Ntcp and P-gp were analyzed by Western Blotting.RESULTS:
After high/middle/low dose isoniazid administration, the expression of Mrp2, Bsep, P-gp and Ntcp were all changed, especially the high/middle dose group. In addition, the biochemical and pathological were significantly lagged behind the expression change of the transporters.CONCLUSION:
The hepatotoxicity of isoniazid may be associated with excessive hepatic accumulation of the related exogenous substances that medicated by Mrp2, Bsep, P-gp and Ntcp.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
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