Preparation of pueratin/MCM-41 assemblies and pharmacokinetic study in rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To synthesize MCM41 mesoporous molecular sieves, prepare pueratin/MCM 41 assemblies, and study their pharmacokinetics in rats. METHODS: MCM-41 mesoporous molecular sieves were synthesized using TEOS as silicon source and CTAB as templates under basic condition. Pueratin was loaded into MCM-41 by immersion method. Pueratin-loaded MCM-41 (PU-MCM) was characterized by X-ray diffraction (XRD), N2 adsorption-desorption and FT-IR spectroscopy. Pharmacokinetic parameters were calculated from the plasma concentrations determined by HPLC after administration of pueratin suspension (PU-SUS) and PU-MCM orally to rats. RESULTS: Pueratin was loaded into the pores of MCM-41 successfully with a drug loading rate of 12.6%. The pharmacokinetic parameters in rats after administration of PU-SUN and PU-MCM were as follows ρmax were (2.43 ± 0.75) and (3.98 ± 1.15) μg · mL-1, tmax were (0.79 ± 0.19) and (0.67 ± 0.20) h, and AUC0~t were (5.35 ± 1.42) and (10.41 ± 2.64) μg · h · mL-1, respectively. CONCLUSION: PU-MCM was prepared successfully, which showed significantly increased release rate, longer residence time and higher bioavailability than PU-SUS after administration in rats.