Determination of monosaccharide composition of polysaccharide in Ganoderma lucidum spore by ion chromatography / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method for determination of monosaccharide composition of Ganoderma lucidum spore polysaccharide. METHODS: Ganoderma lucidum spore polysaccharide was extracted in boiling water and hydrolyzed by 2 mol · L-1 TFA. The monosaccharides were determined by ion chromatography on a CarboPac PA10 column, using 2.5 mmol · L-1 KOH as mobile phase. The column temperature was 30°C. The flow rate was 1.0 mL · min-1. RESULTS: In the certain range, the concentration of each monosaccharide had good linearity with its chromatographic peak area. The recoveries of monosaccharides ranged from 85.6% to 97.0% with RSDs less than 3%. The results showed that Ganoderma lucidum spore polysaccharide was composed of glucose, galactose, mannose, xylose, arabinose, fucose, etc. CONCLUSION: The proposed method has good separation effect and high sensitivity, which can be used for the determination of monosaccharide composition of Ganoderma lucidum spore polysaccharide.