Simultaneous determination of four flavonoid glycosides in Mentha haplocalyx Briq. by a quantitative analysis of multi-components by single marker / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a quantitative analysis of multi-components by single marker (QAMS) method for simultaneous determination of four flavonoid glycosides in Mentha haplocalyx Briq. by using one reference substance. METHODS: Four main effective components (hesperidin, diosmin, didymin, and buddleoside) were selected as analytes to evaluate the quality of Mentha haplocalyx Briq.. With hesperidin as internal reference standard, the relative correction factors (RCF) of the other three components to hesperidin were calculated within certain ranges. The contents of hesperidin in the samples of Mentha haplocalyx Briq. were determined by using the external standard method, and the contents of the three other ingredients were calculated by their RCFs. The method was evaluated by comparison of the quantitative results between external standard method and QAMS method by SPSS 17.0. RESULTS: No significant differences were observed between the quantitative results of these two methods (RSD 0.05). The validated HPLC method had the advantages of precision, reproducibility, and reliability. CONCLUSION: The QAMS method we established is suitable and feasible for the quality control of Mentha haplocalyx Briq.