Optimization of immobilized effect of the O-carboxymethyl chitosan multi-hemostatic sponge on thrombin by the central composite design-response surface methodology / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 138-142, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859873
ABSTRACT
OBJECTIVE:
To investigate the effects of O-carboxymethyl chitosan (O-CMC) multi-hemostatic sponge on the immobilization of thrombin.METHODS:
The formulation of O-CMC multi-hemostatic sponge was optimized by using central composite design-response surface methodology (CCD-RSM), in which the O-CMC, glutaraldehyde and thrombin were used as enzyme immobilization carrier, crosslink agent and coagulant, respectively. The crosslinked conditions of thrombin were studied by determining the enzymatic activity yield.RESULTS:
The optimized concentrations of O-CMC, thrombin and glutaraldehyde were 2.49%, 49.87 U · mL-1, 0.49%, respectively.CONCLUSION:
O-CMC multi-hemostatic sponge has the positive immobilization effect on thrombin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS