Simultaneous determination of seven components in Fangfengtongsheng pills by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop an HPLC method for simultaneous determination of seven components in traditional Chinese medicines Fangfengtongsheng pills from different factories. METHODS: The chromatographic analyses were carried out on a kromasil C18 column(4.6 mm × 250 mm, 5 μm); and the mobile phase was methanol(A)-0.5 acetic acid at 1.0 mL · min-1 in gradient elution mode which was 15% A from 0 to 10 min, 15%-30% A from 10 to 15 min, 30%-40% A from 15 to 40 min, 40%-70% A from 40 to 80 min, and 70%-90% A from 80-120 min; the column temperature and detection wavelength were set at 25°C and 280 nm, respectively. RESULTS: Seven components were determined by HPLC, including baicalin, wogonoside, baicalein, wogonin, chrysin, emodin, and chrysophanol. The recoveries were 101.3%, 98.6%, 99.7%, 96.4%, 102.7%, 100.2% and 98.4%, respectively, and the linearity were good (r ≥ 0.9992) in the concentration ranges of 2.33-300.0, 0.407-120.0, 1.563-50.0, 0.625-40.0, 0.625-77.8, 1.563-100, and 1.625-120 μg · mL-1. CONCLUSION: Seven components are analyzed in Fangfengtongsheng pills by RP-HPLC with gradient elution, and the method has good specificity, reproducibility and high sensitivity, which can be applied to control the quality of Fangfengtongsheng pills.