Chemical constituents from the leaves of Rehmannia glutinosa Libosch / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the chemical constituents in the leaves of Rehmannia glutinosa Libosch. METHODS: The compounds were isolated and purified by Diaion HP-20, Toyopearl HW40, Sephadex LH-20, MCI Gel CHP-20, and silica gel column chromatography, and the structures were elucidated on the basis of spectral data and physiochemical properties. RESULTS: Twenty-seven compounds were obtained from the 50% acetone extract, and their structures were identified as p-hydroxybenzoic acid(1), gentisic acid(2), 6, 7-dihydroxycoumarin(3), p-hydroxyphenylethyl alcohol(4), 3, 4-dihydroxyphe-nylethyl alcohol(5), protocatechuic acid(6), 1, 2, 4-trihydroxybenzene(7), diosmetin(8), luteolin-7-O-β-D-glucuronide(9), apigenin(10), 2β, 3β, 19α-trihydroxyolean-12-ene-13, 28-dioic acid(11), 2α, 3β-dihydroxyolean-12-ene-28-oic acid(12), glutinolic acid(13), β1-hydroxyacteoside(14), echinacoside(15), jionoside B,(16), acteoside(17), isoacteoside(18), ajugol(19), catalpol(20), 8-epiloganic acid(21), luteolin(22), oleanolic acid(23), ursolic acid(24), oleanonic acid(25), β-sitosterol(26) and daucosterol(27). CONCLUSION: Compounds 1-14 and 23-25 are isolated from this plant for the first time.