Screening of macromolecules in traditional Chinese medicine injections / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 64-67, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-859893
ABSTRACT
OBJECTIVE:
To establish methods for screening of macromolecules in traditional Chinese medicine injections by molecular exclusion chromatography (SEC).METHODS:
The separation was carried out by SEC with Phenomenon BioSep-SEC-s2000 (7.8 mm×300 mm, 8 μm) column. The mobile phase was 0.05 mol·L-1 Na2SO4, and differential detector was used when dextran was used as the reference standard. When proteins were used as reference standards, the mobile phase was the mixture of 0.1% trifluoroacetic acid and acetonitrile (70-30), and UV detector was selected.RESULTS:
With the differential detector, the molecular weight range of dextran was 7100-133800, and the limit of detection was 0.0979 μg; with the UV detector, the molecular weight range of the proteins was 1638-13700, and the limit of detection was 0.00358 μg. No macromolecules were detected in the selected traditional Chinese medicine injections.CONCLUSION:
The proposed method can be used for the screening of macromolecules in traditional Chinese medicine injections.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
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