Sonication Induces Apoptosis in HL-60 Cells / 계명의대학술지
Keimyung Medical Journal
;
: 109-119, 2014.
Article
in Korean
| WPRIM
| ID: wpr-85996
ABSTRACT
To investigate a specific mechanism of apoptosis induced by sonication, we applied 20 kHz ultrasound to leukemia cell line HL-60 with different intensities (0-60 W/cm2) and time durations (0-100 sec). In accordance with previous reports, ultrasound treatment in HL-60 cells induced immediate cell death and delayed cell death which are associated with cell lysis and apoptosis, respectively. Delayed cell death of HL-60 was also detected 5 hours after sonication in our experiment. Detection of caspase activation by Western blot and sub-G1 accumulation by flow cytometry confirmed that apoptosis plays a role in delayed cell death induced by sonication in HL-60 cells. In addition, we found that decrease in lysosomes of HL-60 cells after sonication suggesting lysosomal rupture is involved in the mechanism of cell death induced by sonication.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rupture
/
Sonication
/
Ultrasonic Therapy
/
Leukemia
/
Cell Line
/
Blotting, Western
/
Ultrasonography
/
Cell Death
/
Apoptosis
/
HL-60 Cells
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Keimyung Medical Journal
Year:
2014
Type:
Article
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