Two-way effects on nasopharyngeal carcinoma CNE2 cells growth and mechanisms of different dose naringenin / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the influence and the mechanisms of different dose naringenin on nasopharyngeal carcinoma CNE2 cells growth. METHODS: Different concentrations of naringenin (0, 5, 10, 20, 40, 60, 80, 100, 200, 400, 800 μg · mL-1) were used to detect their effects on CNE2 cells growth for 24 to 72 h by MTT Method. High concentrations of naringenin (0, 200, 400 μg · mL-1) for 48 h were used to detect apoptosis by Hochest 33 258 staining. High concentrations of naringenin (0, 200, 400 μg · mL-1) for 48 h were used to detect the cell cycle by flow cytometry. Different concentrations of naringenin (0, 40, 200 μg · mL-1) for 24 and 48 h were used to detect the level of reactive oxygen species (ROS) by flow cytometry, and mRNA expression levels of P53 and C-fos were determined by qPCR. RESULTS: After different concentrations of naringenin administration, CNE2 cells were changed growth rate. Relatively low dose can induce the low level rise of ROS, the low level rise of ROS can induce the up-regulation of the expression levels of c-fos (P < 0.01) and the down-regulation of the expression levels of P53(P < 0.01), so relatively low dose can promote CNE2 cells growth rate. However, relatively high dose can induce the high level rise of ROS, the high level rise of ROS can induce the up-regulation of the expression levels of P53(P < 0.01) and the down-regulation of the expression levels of C-fos (P < 0.01), so relatively high dose can induce apoptosis, also possess the effect of S and G2/M phase blockage(P < 0.01), significantly suppress CNE2 cells growth rate. CONCLUSION: Different dose naringenin has two-way effects on CNE2 cells growth, relatively low dose has carcinogenic effect, relatively high dose has good anticancer effect.