Determination of anthraquinones in Dabaidu capsules by quantitative analysis of multi-components by single marker / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 2039-2044, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-860138
ABSTRACT
OBJECTIVE:
To establish a auantitative analysis of multi-components by single marker(QAMS) method for determining the content of anthraquinones in Dabaidu capsules(traditional Chinese medicines).METHODS:
The relative correction factors (RCFs) of emodin to rhein, chrysophanol, and physcion were determined on a C18 column (4.6 mm × 250 mm, 5μm) with methanol-0.1% phosphoric acid (8515) as mobile phase. The flow rate was 1.0 mL · min-1. The column temperature was set at 25°C. The detection wavelength was set at 254 nm. The relative retention time (RRT) was used to define the target peak.RESULTS:
The calibration curves for rhein, emodin, chrysophanol, and physcion showed good linearity in the ranges of 5.724 × 10-2-0.5724 μg, 4.584 × 10-2 -0.4584 μg, 6.368 × 10-2-0.6368 μg and 6.548 × 10-2 - 0.6548 μg, r(0.9997-0.9999), respectively. The recoveries were (97.8 ± 1.4)%, (100.7 ± 1.0)%, (98.6 ± 0.6)% and (97.0 ± 1.4)%, respectively. The RCFs were 1.19, 1.40 and 1.06.CONCLUSION:
The method is simple, rapid, accurate and reliable. It can be applied to control the quality of anthraquinones in Dabaidu capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS