The effect on miRNAs of replant liver cancer rats treated by Aitongxiao granule / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the effect of Aitongxiao granule (traditional Chinese medicines) therapy on miRNAs of replant liver cancer rats. METHODS: The 60 replant liver cancer rats were randomly divided into high dose(A), middle dose(B), lower dose(C), 5-Fu(D), distilled water(E) groups. After 14 days of experimental treatment, the expressions of miR-199a-3p,miR-199a-5p, miR-18 in liver cancer tissues were detected with qRT-PCR. RESULTS: Before experimental treatment, in comparison with E group, the level of miR-18 was higher but level of miR-199a-3p, miR-199a-5p were lower in A,B,C,D groups(P < 0.01). After 14 days of experimental treatment, in comparison with E group, the level of miR-18 was lower but level of miR-199a-3p, miR-199a-5p were higher in A,B,C,D groups(P < 0.01). The more significant differences were found in B group than that in D group. CONCLUSION: The Aitongxiao granule has the regulative effects on miRNAs of replant liver cancer rats. Its therapeutical effects relate to the miRNAs expressions of liver cellular cancer development.