Establishment of HPLC speciftic chromatogram of Dendrobium officinale capsules / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1660-1663, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-860213
ABSTRACT
OBJECTIVE:
To establish the RP-HPLC specific chromatogram of Dendrobium officinale capsules for the quality control.METHODS:
An Ultimate C18 column (4.6 mm × 250 mm, 5 μm) maintained at 30°C was used for all analysis. Chromatographic separation was carried out using gradient elution of methanol and 0.2% phosphoric acid aqueous solution at a flow rate of 1.0 mL · min. The detection wavelength was set at 280 nm. The similarity evaluation system for chromatographic fingerprint of TCM (National Pharmacopoeia Committee) was adopted to construct the specific chromatogram.RESULTS:
The HPLC specific chromatogram of Dendrobium officinale capsules was constructed with four common specific peaks with naringenin peak as the reference.CONCLUSION:
The method shows good precision and repeatability. It can be used for the quality control of Dendrobium officinale capsules and can be taken as a reference for the identification and quality control of other Dendrobium officinale-related health foods.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Type:
Article
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