Biocontrol of Dendrobium candidum damping-off disease with endophytic fungi / 中国药学杂志

ABSTRACT

OBJECTIVE: To screen out endophytic fungi isolates with good biological control against damping-off in Dendrohium candidum seedlings among the isolates owned by our laboratory, thus to lay a foundation on developing biological control technique and field application. METHODS: Eighteen isolates of endophytic fungi identified by the PDA Petri dish confrontation experiment and athogen of damping-off were used in the biological control experiment. Co-incubation in bottles was conducted to screen out isolates with weak pathogenic and growth promoting effect on Dendrobium candidum seedlings. The chosen isolates were then enrolled in the potted seedlings experiment to determine their biological control effect. RESULTS: Isolates 4829 and 3952 were confirmed to have good biological control effect by statistical analysis. The survival rates of Dendrobium candidum seedlings reached 66.7% and 60.5% respectively. CONCLUSION: Endophytic fungi isolated from Dendrobium sp. shows good biological control against Dendrobium candidum damping-off.