Protective effect of tanshinone II a on myocardial apoptosis in rats with sepsis / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the effect of tanshinone II A (Tan II A) on the myocardial apoptosis in rats with sepsis. METHODS: The sepsis rat model was established by cecal ligation and puncture operation (CLP), and the Tan II A was given for 12 consecutive hours. Then, the following indices were measured such as the activities of Na+-K+-ATPase and SOD, the contents of TNF-α, IL-1β, calcium, MDA, apoptotic index and the protein level of Bcl-2, Bax, Fas, caspase-3, calcincurin in myocardial. RESULTS: CD Compared with sham group, CLP treatment can result in decreased myocardial activities of Na+-K+-ATPase and SOD, elevated the contents of IL-1β, calcium, MDA (P<0.05); Tan II A treatment can improve the above aberrant indices. (2) Compared with sham group, CLP treatment can elevated the myocardial apoptotic index (P<0.05), and Tan II A treatment can decrease the elevation of apoptotic index by CLP treatment (P<0.05); (3)Compared with sham group, the protein level of Bax, Fas, caspase-3 and calcincurin significantly increased, and protein level of Bcl-2 decreased (P<0.05) in CLP group, and Tan II A treatment can improve exceptional expression of the above proteins (P<0.05). CONCLUSION: Tanshinone II A shows a protective effect on the myocardial apoptosis in septic rats maybe by depressing inflammatory infiltration and oxidative stress reaction, relieving calcium overload and partly improving the exceptional expression of the proteins such as Bax, Bcl-2, Fas, caspases-3 and calcincurin.