Comparative study on the differentiation and maturation of rat calvarial osteoblasts by naringin and Na-ringenin in vitro / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effects on the differentiation and maturation of rat osteoblast (ROB) by naringin and it's metabolite-naringenin. METHODS: Primary ROB were obtained from new born SD rat skull after dissected and digested many times by type II collagenase during sterile condition. Serial subcultivation were proceeded when cells covered 80% culture dish. Cell proliferation was detected by MTT, while the alkaline phosphatase (ALP) activity was adopted as osteogenic differentiation marker to screen the best concentration of naringin and naringenin. The secretion of osteocalcin, bone morphogenetic protein-2 (BMP-2), oes-teopontin(OPN) and collagen I, the bone mineralized nodulus, even the gene expression of bFGF, IGF-1, Runx-2, Osterix, OPG and RANKL all were compared among the naringin-supplemented group, naringenin-supplemented group and the control. RESULTS: Both naringin and naringenin can significantly improved ALP activity, the secretion of osteocalcin, BMP-2, OPN and collagen I, the bone mineralized nodulus also were raised. Besides, these two drugs also stimulated the expression of genes which related to the osteogenesis of ROB. However, naringenin is stronger than naringin in above markers significantly. CONCLUSION: The osteoprotective effects of naringenin is stronger than naringin at enhancing the osteogenic differentiation of ROB, suggesting that naringin can be administered via oral and its metabolites developed higher activity to prevent osteoporosis. These results may provide a guide for the new drug develop and dosage forms design during osteoporosis therapy.