Simultaneous determination of seven components in Jinlianqingre granules by HPLC-MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method for the determination of orientin, vitexin, hyperin, neomangiferin, mangiferin, timosaponin BII and timosaponin AIII in Jinlianqingre granules(traditional Chinese medicines) by HPLC-MS. METHODS: The samples were separated on a Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) by gradient elution using acetonitrile and 0.1% aqueous formic acid as the mobile phase at a flow rate of 800 μX · min-1. The column temperature was 30°C. Multiple-reaction monitoring (MRM) scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. RESULTS: Complete separation was obtained within 15 min for the seven compounds. The regression equations showed linear relationships between the peak area and content of each compound (r > 0.9992). The average recoveries of the compounds listed above were 99.34%, 99.28%, 101.6%, 101.3%, 100.5%, 101.9% and 98.86% (n = 3), and the RSDs were 1.12%, 1.36%, 1.57%, 1.74%, 1.52%, 1.43% and 0.92%, respectively. CONCLUSION: The method is simple, accurate and highly reproducible, and can be used for the determination of seven compounds in Jinlianqingre granules.