Dose-response and HPLC spectrum-activity relationships of the anti-inflammatory active site of Kalimeris indica / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the dose-effect and fingerprint-effect relationships of the active site of Kalimeris indica. METHODS: The HPLC fingerprints of the active site at different doses were determined. Mouse auricle swelling model was used to observe the anti-inflammatory action of Kalimeris indica extract and its dose-response relationship. Grey correlation method was used to assess the relationship between the fingerprint peaks and effects. RESULTS: The was a positive correlation between the dose and anti-inflammatory activity of the extract (r=0.9511). The extract had 18 common peaks, among which four were identified, and the comprehensive correlation between the common peaks and anti-inflammatory activity was from 0.5608 to 0.9599, with ranking of 10> 14> 5> 8> 15> 9> 2> 7> 12> 1> 17> 3> 18> 16> 6> 13> 4> 11. CONCLUSION: The spectrum-activity relationship of Kalimeris indica established in this study could provide theoretical basis and data support for the quality control pattern of traditional Chinese medicines.