Establishment of stereospecific assay of flurbiprofen by high-performance liquid-chromatographic tandem-mass spectrometry and its application to pharmacokinetic study / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop an enantioselective HPLC-MS-MS method for the pharmacokinetic study of flurbiprofen transdermal patches. METHODS: The analyte and internal standard, ketoprofen, were extracted from plasma by methyl tert-butyl ether, analyzed on a Chiralpak AD-RH column(4.0 mm×150 mm, 5 μm)with mobile phase consisting of acetonitrile-ammonium acetate buffer solution(2 mmol·L-1 pH 5.3, 5050, V/V), and detected with UV detector at 273 nm. RESULTS: The assay was linear over the concentration range of 2.0-1000 ng·mL-1 and the intra- and inter-day precision over this range were not more than 5.8%. The limit of quantification in plasma was 2.0 ng·mL-1. CONCLUSION: The method established here is accurate, precise, rapid, sensitive, and successfully applied to the enantioselective determination of flurbiprofen in the pharmacokinetic study of flurbiprofen transdermal patches in rabbits.