ITS sequences and phylogenetic relationships of dandelions from different areas / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 687-690, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-860392
ABSTRACT
OBJECTIVE:
To study the genetic characteristics and phylogenetic relationships of dandelions of 20 populations by sequencing ITS sequence.METHODS:
The ITS sequence was amplified by PCR with universal primer of ITS, and the ITS sequence of the PCR products was directly sequenced after purification.RESULTS:
The total length of ITS sequence of different samples was 711 bp. The ITS sequence was divided into three fragments; ITS1 was 225 bp, 5.8S was 262 bp and ITS2 was 226 bp. In all samples 5.8S was comparatively conserved, while ITS1 and ITS2 had higher diversity in base sequence among the various specimens.CONCLUSION:
ITS sequence can be used to analyze the phylogenetic relationships of different varieties of dandelions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Type:
Article
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