Establishment of a quantitative method for tetramethylpyrazine hydrochloride in rat plasma using pyramide as internal standard / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 730-732, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-860401
ABSTRACT
OBJECTIVE:
To develop an HPCE method of tetramethylpyrazine hydrochloride in rat plasma using pyramide as the internal standard.METHODS:
The separation was performed on a fused-silica uncoated capillary with an inner diameter (ID) of 75 μm, an effective length of 50 cm and a total length of 60 cm. The optimum HPCE conditions were as follows 100 mmol·L-1 SDS-30 mmol·L-1 sodium tetraborate solution (4060) was used as the background electrolyt, the samples were injected at the anodic end at 0.5 psi for 6 s, separation voltage was 20 kV at positive power, capillary temperature was 20°C, and detection wavelength was 295 nm.RESULTS:
The linear range was 1.12-286.72 mg·L-1(r=0.9996), and the inter-day and intra-day precisions expressed as RSDs were less than 4.21% and 4.74%, respectively. The average recoveries were 91.22%-97.48%, and the limit of detection was 0.16 mg·L-1.CONCLUSION:
This method is rapid and accurate to quantify tetramethylpyrazine hydrochloride in rat plasma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Type:
Article
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