Determination of propofol in follicular fluid by HPLC / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 743-745, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-860404
ABSTRACT
OBJECTIVE:
To develop a sensitive and specific reverse-phase high-performance liquid chromatography (RP-HPLC) method to determine propofol in follicular fluid.METHODS:
Thymol was added into follicular fluid samples as the internal standard, and the samples were then precipitated with methanol. After centrifugation at 20000 r·min-1 for 15 min, 20 μL of supernatant was directly injected into an Agilent Extend-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was composed of methanol-water (7723). Analysis was run at a flow rate of 1.0 mL ·min-1. Fluorescence detection was applied with excitation and emission wavelengths of 276 and 310 nm, respectively.RESULTS:
The calibration curves had good linearity in the range of 0.108-5.4 mg·L-1. The linear regression equation was A=0.2074ρ-0.0012(r=0.9993). The limits of detection and quantity proved to be 54 and 108 μg·L-1, respectively. The method recoveries for low, intermediate and high concentrations were 117.9%, 101.7% and 99.4%, respectively. The RSDs of inter-day assay were 4.10%, 1.28% and 0.20%(n=5), and intra-day assay were 4.34%, 2.33% and 0.61%(n=5), respectively.CONCLUSION:
The method is accurate and convenient. It can be used to determine propofol for studying its clinical pharmacokinetics.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Type:
Article
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