Primary study of Dendrobium polysaccharides improving immunity activity on mouse / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the effect on mice immunity activity of Dendrobium devonianum polysaccharides and Dendrobium candidum polysaccharides. METHODS: Cultured splenic lymphocytes of mice in vitro to observe the influence of Dendrobium devonianum polysaccharides and Dendrobium candidum polysaccharides separately cooperated with ConA on proliferation of splenic lymphocyte and content of IFN-γ and IL-2. In animal experiments, respectively offered Dendrobium devonianum polysaccharides and Dendrobium candidum polysaccharides to mouse in different dosage, after 14d, copied the model of toe swelling induced by SRBC to observe the effect of Dendrobium polysaccharides on toe swelling and viscera index. RESULTS: Medium dosage groups of two Dendrobium polysaccharides both can stimulate the proliferation of splenic lymphocyte observably; medium and high dosage groups both can promote the secretion of IFN-γ and IL-2 in splenic lymphocyte In addition, high dosage groups of two Dendrobium polysaccharides both can increase toe swelling and spleen index of mouse obviously, but every dosage groups have no effect on thymus index of mouse. CONCLUSION: To some extent, Dendrobium devonianum polysaccharides and Dendrobium candidum polysaccharides can improve the immunity activity of mouse.