Determination of amifostine in human saliva by HPLC-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an HPLC-MS/MS method (high performance liquid chromatography-tandem mass spectrometry) for the direct determination of amifostine in human saliva. METHODS: Saliva samples were collected from six adult healthy volunteers. After protein precipitation and addition of the internal standard (IS) huperzine-A (HupA), HPLC-MS/MS was used to analyze amifostine. The analysis was conducted using a ZIC-HILIC analytical column (2.1 mm × 100 mm, 3.5 μm). Electrospray ionization was used with multiple reaction monitoring (MRM) mode. RESULTS: The lower limit of quantification (LLOQ) of the method was 0.938 mg · L-1 (S/N<10). The standard curve was linear in the range of 0.938-30 mg · L (r=0.9991, n=6). The inter-day and intra-day RSDs were all less than 15% for the low, medium and high concentration quality control samples (1.0, 5.0 and 25 mg · L-1) The values of recovery were all more than 85%. CONCLUSION: The method is direct, rapid, simple and sensitive, and suitable for the determination of amifostine in saliva samples.