Inhibitory effect of platycodin D on human colonic cancer SW620 cell proliferation / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the inhibitory effect and its mechanism of platycodin D (PD) on human colonic cancer SW620 cell proliferation. METHODS: The inhibitory effect of PD on SW620 cell proliferation was analyzed by MTT assay, while the effect of PD on cell cycle distribution and apoptosis were evaluated by flow cytometry. The effect of PD on expression of cyclin and ap-optosis associated proteins were detected by Western blot. RESULTS: The cell proliferation of human colonic cancer SW620 cell was inhibited by PD in a dose-dependent manner. After cells were treated with PD(15, 20 μmol · L-1) for 24 h, the proportions of cells in G0/G1 phase were (72.83±5.26)% and (76.82±5.83)% respectively, while the control group cells was (56.78±4.92)%, which suggested PD could block SW620 in the G, phase compared with the control group cells. Furthermore, the expressions of cyclinD1, c-myc and GDK6 were reduced obviously. Compared with the control group cells, the apoptotic rates were increased [(19.5±5.1)%, (35.8±5.3)% and (43.8±4.0)% respectively] after cells were treated by PD (10, 15, 20 μmol · L-1) for 48 h. The expression of procaspase 3 and PARP with proenzyme form were reduced. CONCLUSION: PD could inhibit the growth of colon cancer cell by blocking SW620 in the G, phase through regulating the expression of cyclinD1, c-myc and CDK6 and thus inducing the apoptosis by cell cycle arrest.