Proliferation inhibition and apoptosis induction effect of dextran-magnetic layered double hydroxide-fluorouracil drug delivery system on human colon cancer cells SW480 / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the proliferation inhibition and apoptosis induction effect of dextran-magnetic layered double hydroxide-fluorouracil (DMF) drug delivery system on colon cancer cell SW480 cultivated in vitro. METHODS: MTT experiment was used to detect the cell proliferation inhibition rates of DET-MLDH-FU, MLDH-FU and MLDH supra-molecules at various concentrations. Light microscopy and Giemsa dyeing methods were applied for characterizing the shape change of the apoptotic cells. Flow cytometric analysis was employed to test the apoptosis rates of SW480 cells treated with different drugs for 24 h. DNA ladder method was used to analyze nuclei breakup of the apoptosis cells. RESULTS: The IC50 of MLDH, MLDH-FU and DMF three-level supra-molecules for SW480 cells were 44.83(25.74, 48.78), 15.16(13.78, 16.66) and 13.33 (11.03, 15.13) μg · mL-1, respectively. The proliferation of colon cancer cells SW480 was obviously restrained after being treated with 5-Fu and equivalent MLDH, MLDH-FU and DMF of different concentrations for 24, 48 and 72 h, respectively. The result revealed that the inhibition rates increased in the sequence of MLDHdose-effect relationship and time dependence. Compared with FU, DMF eausefl significantly different proliferation inhibition and early apoptosis of SW480 cells. In addition, DMF also caused agglutination of intra-nuclear chromatin and disintegration of cells.

CONCLUSION:

With low cytotoxicity, MLDH can be used as a drug carrier. Compared with FU, 2-3 grades supra-molecular assembly of MLDH-FU and DET-MLDH-FU have relatively higher transmission efficiency and produces significant proliferation inhibition and apoptosis induction effect for SW480 cells.