Isolation, identification and antimicrobial activities of endophytic fungi from Tibetan drug Lycium ruthenicum Murr / 中国药学杂志

ABSTRACT

OBJECTIVE: To isolate and identify endophytic fungi from the Tibetan drug Lycium ruthenicum Murr., then to investigate their antimicrobial activities, in order to obtain the strain with strong antimicrobial activities. METHODS: Endophytic fungi were isolated from the roots, stems and leaves of Lycium ruthenicum Murr., and then morphologically identified, and the antimicrobial activities were investigated by paper sheet method. RESULTS: Eighty-one strains of endophytic fungi were isolated from Lycium ruthenicum Murr., of which 60 strains were isolated in the general condition, 21 strains in high level of salinity. The 81 strains were i-dentified as 7 orders, 10 families, and 13genuses. The experiments showed saline-alkali fungus had stronger antimicrobial activities than the strains isolated in the general condition. CONCLUSION: It is very essential to simulate the growth environment of plants when isolating endophytic fungi.