Determination of proanthocyanidins and total phenolics in the seedpod of Nelumbo nucifera Gaertn by near infrared diffuse reflectance spectroscopy / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a method for rapid determination of proanthocyanidins and total phenolics in the seedpod of Nelumbo nucifera Gaertn by near infrared diffuse reflectance spectroscopy. METHODS: Calibration models were established using PLS (partial least squares) method, while the contents of proanthocyanidins and total phenolics were measured by UV. RESULTS: The range of 7500-6900 cm-1 of near infrared spectra was selected. Different spectra pretreatment methods were compared, and it was shown that second derivative pretreatment and Savitzky-Golay smoothing filter methods could extract spectra information thoroughly. The correlation coefficient (r) of proanthocyanidins was 0.9621, SEC (st andard deviation of the calibration sets) was 0.64, and SEP (st andarddeviation of the prediction sets) was 0.94. The correlation coefficient (r) of total phenolics was 0.9254, SEC was 0.97, and SEP was 1.26. CONCLUSION: The near infrared diffuse reflectance spectroscopy method can be used to analyze the main active components in the seedpod of Nelumbo nucifera Gaertn rapidly, so it can be used routinely by the manufacturers for the quality control of the raw materials.