Analysis of lignans in Shengmai injection by HPLC-MS/MS / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 132-135, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-860503
ABSTRACT
OBJECTIVE:
To establish a determination method of lignans in Shengmai injection.METHODS:
On the basis of the study on the chemical constituents of Schisandrae Chinensis Fructus extracts, the peaks in the chromatograms of Shengmai injections from eight production enterprises were identified by HPLC-MS/MS. Agilent 6320 Ion Trap LC/MS, the separation was carried out by using an Waters symmetry shield TM RP18 column (4.6 cm × 25 cm, 5 μm), with a gradient mobile phase of acetonitrile-ammonium acetate water solution, detector wavelength was 203 nm; MS parameters; nebulizer 2 kPa; dry gas 12 mL · min-1; dry temperature 350°C; collision pressure 1.0 V; positive ion scan.RESULTS:
By comparing the Schisandrae Chinensis Fructus extracts and the Shengmai injection, it indicated that the lignans is the primary component in Schisandrae Chinensis Fructus extracts, and the mutual ingredients of the 8 enterprises is schizandrol A, gomisin D, gomisin J and angeloylgomisin Q.CONCLUSION:
The established HPLC-MS/MS method can be used as the basis for further study and quality control of lignans in Shengmai injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Type:
Article
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