Pharmacokinetic interaction between aminophylline and metronidazole in rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an HPLC-UV method for the simultaneous determination of theophylline and metronidazole in rat plasma, so as to explore the pharmacokinetic interaction between theophylline and metronidazole. METHODS: Eighteen male Sprague-Dawley (SD) rats were divided into aminophylline group, metronidazole group, aminophylline and metronidazole group. Blood samples were collected at intervals after each intervenous administration. Then the concentrations of theophylline and metronidazole were determined by HPLC, and the pharmacokinetic parameters were calculated by DAS2.0 software and evaluated by one-way analysis of variance using SPSS19.0 software. Drug-drug interaction was tested by 95% confidence interval. RESULTS: Compared with single mediacation groups, the pharmacokinetic parameters t1/2 MRT, AUC0-t, Vd and CL of the combined group showed no significant difference (P > 0.05). CONCLUSION: Aminophylline and metronidazole do not have pharmacokinetic interaction in rats. Copyright 2013 by the Chinese Pharmaceutical Association.