High throughput screening and detection of peptaibol antibiotics from the fermentation broth of tri-choderma harzianum by LC-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify peptaibol antibiotics from the fermentation broth of Trichoderma harzianum CGMCC No. 1780(China General Microbiological Culture Collection). METHODS: Peptaibol antibiotics were isolated using a bioassay-guided fractionation against Rhizoctonia solani. To purify the peptaibols, the fermentation broth was fractionated by polystyrene resin, sephadex and high performance thin-layer chromatography (HTLC). A specific chromogenic reaction with 4, 4'-methylenebis (N, N-dimethylani-line) was used for the preliminary identification of peptaibols. The sequences of the peptaibol antibiotics were determined by LC-MS/MS analysis. RESULTS: Twenty peptaibol antibiotics were identified from the fermentation broth of T. harzianum and five of the peptaibols were novel. CONCLUSION: The fermentation broth of T. harzianum is a rich source of peptaibol antibiotics warranting further study and the LC-MS/MS method is rapid and effective to analyze the peptaibols.