Determination of the activity of PEOZ liposomal SOD mimic using NBT-Illumination method / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the method for determinating the activity of [poly(2-ethyl-2-oxazoline), PEOZ]-modified liposomes encapsulated superoxide dismutase (SOD) mimic (PEOZ-L-SOD). METHODS: PEOZ-L-SOD was prepared by film dispersion method, and the activities of the SOD mimic and PEOZ-L-SOD were determined by NBT-Illumination method. RESULTS: The fitted equation of the inhibition rate curve of the SOD mimic was IR%=33.4211nρ + 49.715 (r2=0.9992) and the IC50 was 1.0086 × 10-3 μmol · L-1; the fitted equation of the inhibition rate curve of PEOZ-L-SOD was IR%=33.5211nρ + 49.671 (r2=0.9991) and the IC50 was 1.0099 × 10-3 μmol · L-1. CONCLUSION: The NBT-Illumination method is simple, reliable, economic and practical, and can be used as an efficient method to determine the activity of SOD mimic liposomes. The experiment proves that the activity of SOD mimic did not change after it was encapsulated in the liposomes.