In vitro and in vivo microdialysis recovery of tetramethylpyrazine / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the relationship between flow rate and drug concentration and the recoveries of microdialysis probes. METHODS: Tetramethylpyrazine (TMP) was selected as the model drug. The in vitro recovery of TMP was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations. And the in vivo recovery of TMP was studied using retrodialysis method. RESULTS: The microdialysis recoveries of TMP were inversely proportional to the perfusing rates while independent of TMP concentrations. The recoveries of positive dialysis were quite different from that of retrodialysis in vitro. While the recovery of in vitro positive dialysis was similar to that of in vivo retrodialysis. CONCLUSION: Microdialysis is a useful and reliable tool to study the pharmacokinetics of TMP and in vivo retrodialysis method can be used for TMP pharmacokinetics.